Application of Quantitative NMR Spectroscopy to the Quality Evaluation of Diclofenac Gargles as Hospital Preparations

Hospital preparations are frequently prepared in Japanese hospitals when ready-made formulations to meet patients’ needs unavailable. Although the quality of hospital have be ensured for efficacy and safety, such evaluation tends insufficient mainly due lack manpower experimental environments hospitals. In this paper, we investigated applicability quantitative (q)NMR spectroscopy control diclofenac gargles as examples preparations, it has various merits analysis mixtures solutions. Diclofenac composed diclofenac, tranexamic acid, lidocaine, used pain relief stomatitis induced by cancer chemotherapy. Aliquots gargles, which were five times, mixed with dimethylsulfone an internal standard, followed qNMR measurements. Water signal suppression was achieved using a pulse program, water enhanced through T1 effects, because program superior other ones presaturation one-dimensional nuclear Overhauser effect terms quantitativeness. Concentrations three medicinal ingredients simultaneously determined based on signals selected considering spectral separation Consequently, found constant quality, stable at room temperature least four weeks. is considered potentially useful minimal sample pretreatments, need calibration curves, its comprehensive detection abilities.